Fig. 3: Model fit to dose response data predicts time-series data for multiple topologies. | Communications Biology

Fig. 3: Model fit to dose response data predicts time-series data for multiple topologies.

From: Quantitative characterization of recombinase-based digitizer circuits enables predictable amplification of biological signals

Fig. 3

A Flow chart of modeling signal amplification predictions and how these can be mapped back to find optimal Flp:shRNA ratios given a particular input signal. B Heatmaps highlighting the trends in digitizer fold change demonstrate similar trends between predicted (left) and experimentally observed (right) values for each topology. C Scatter plots of predicted versus actual fold change between plus/minus DOX conditions for each of our three systems at 48 h post transient transfection/induction. Each point represents a different recombinase:shRNA ratio. Number of ratios in each plot is n=16, n=36 and n=36 for the No shRNA, Constant shRNA, and Feedforward shRNA respectively. Pearson correlation coefficient (r) shown at top left of each plot.

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