Fig. 4: Model predicts qualitative enhancement of synNotch signaling by digitizer modules.

A Diagram of the synNotch sensor coupled with each recombinase digitizer topology. B IFP (top, synNotch output), experimental OFP (middle, digitizer output), and simulated OFP (bottom) signals demonstrate improved population distinguishability after the signal is transduced by the digitizer. Model results for each digitizer’s ability to handle the given input signal from the synNotch sensor qualitatively matches each trend. Experimental data (Digitizer Input and Digitizer Output) represent the top 30% of cells expressing a CFP at 48 h post coculture (72 h post transfection).