Fig. 5: RT-qPCR validation of genes coding for enzymes involved in the cholesterol biosynthetic process.

Relative changes in gene expression of genes involved in cholesterol synthesis in the Arabian camel kidney a medulla and b cortex after severe dehydration and acute rehydration compared to controls. Comparison of the means by one-way ANOVA (Turkey’s post hoc correction). The boxplots are presented with the S.E.M. (n = 5), centre lines show median, box edges delineate 25th and 75th percentiles and bars extend to minimum and maximum values. Individual data points represent biologically independent samples and data points within red triangles denote outliers, all the outliers highlighted were included for the statistical analyses. ***padj < 0.001, **padj < 0.01, *padj < 0.05.