Fig. 1: Overview of Tag-seq and its bioinformatics analysis workflow. | Communications Biology

Fig. 1: Overview of Tag-seq and its bioinformatics analysis workflow.

From: Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases

Fig. 1

a Schematic of Tag-seq. It involves five steps: cell transfection, genomic DNA extraction, a single-tube reaction (including fragmentation, end repair, dA-tailing and adapter ligation), PCR amplification, and next-generation sequencing. It spends ~2–3 day + 220 min for libraries preparation. b The Tag-seq data analysis workflow contains three parts: data filtering, editing sites detection, and visualization. c Tag-seq data analysis scheme. It starts with only FASTQ files (R1, R2) and a reference genome.

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