Fig. 5: Tag-seq assesses the specificity of CRISPR.

a–c The specificity of wild-type SpCas9 and two high-fidelity variants (eSpCas9 and SpCas9-HF) were assessed at site EMX1 (a), AAVS1 (b), and CTLA4 (c). d The specificity of the AsCpf1 and LbCfp1 nucleases were assessed at site CCR5.