Table 1 Cryo-EM data collection and data processing statistics.
From: Structural plasticity of mumps virus nucleocapsids with cryo-EM structures
|  | Nring-13p (EMDB-31361) (PDB-7EWQ) | Nring-14p (EMDB-30281) (PDB-7EWQ) | NChelix-dense (EMDB-31368) (PDB-7EXA) | NChelix-hyper (EMDB-31369) (PDB-7EXA) | Nring-stacked (EMDB-31370) (PDB-7EXA) | NChelix-Δarm (EMDB-31367) (PDB-7EXA) |
|---|---|---|---|---|---|---|
Data collection and processing | ||||||
Microscope | Titan Krios G3i | Titan Krios G3i | Titan Krios G2 | Titan Krios G2 | Titan Krios G2 | Titan Krios G2 |
Voltage (kV) | 300 | 300 | 300 | 300 | 300 | 300 |
Camera | Gatan K3 Bio-Quantum | Gatan K3 Bio-Quantum | Gatan K2Â Summit | Gatan K2 Summit | Gatan K2 Summit | Gatan K3Â Summit |
Magnification | 81,000 | 81,000 | 18,000 | 18,000 | 18,000 | 18,000 |
Electron exposure (e−/Å2) | 50 | 50 | 40 | 40 | 40 | 40 |
Defocus range (μm) | 1.5–3 | 1.5–3 | 1.5–3 | 1.5–3 | 1.5–3 | 1.5–3 |
Pixel size (Ã…) | 0.53 | 0.53 | 0.65 | 0.65 | 0.65 | 0.66 |
Symmetry imposed | C13 | C14 | Helical | Helical | C13 | Helical |
Initial particle images (no.) | 1,012,258 | 1,012,258 | 574,262 | 574,262 | 574,262 | 242,861 |
Final particle images (no.) | 390,418 | 84,124 | 45,506 | 38,110 | 79,901 | 192,387 |
Map resolution (Å)  FSC threshold | 3.3 0.143 | 6.2 0.143 | 3.9 0.143 | 3.6 0.143 | 3.7 0.143 | 2.9 0.143 |
Map resolution range (Å) | 3.3–10 | 6.2–15 | 3.6–4.2 | 3.4–3.8 | 3.5–3.9 | 2.4–3 |
Refinement | ||||||
Initial model used (PDB code) | 4XJN | 4XJN | 6JC3 | 4UFT | 6JC3 | — |
Model resolution (Å)  FSC threshold | 3.3 0.143 | 6.2 0.143 | 3.9 0.143 | 3.6 0.143 | 3.7 0.143 | 2.9 0.143 |
Map sharpening B factor (Å2) | −135.99 | −135.99 | −188.62 | −157.92 | −173.72 | −127.25 |
Model composition | ||||||
 Non-hydrogen atoms  Protein residues  Ligands | 3088 374 0 | 3088 374 0 | 2968 374 0 | 2968 374 0 | 2968 374 0 | 2968 374 0 |
B factors (Ã…2) | ||||||
 Protein  Ligand | 164.92 — | 164.92 — | 15.57 — | 15.57 — | 15.57 — | 15.57 — |
R.m.s. deviations | ||||||
 Bond lengths (Å)  Bond angles (°) | 0.011 1.161 | 0.011 1.161 | 0.011 1.161 | 0.011 1.161 | 0.011 1.161 | 0.011 1.161 |
Validation | ||||||
 MolProbity score  Clashscore  Poor rotamers (%) | 2.28 22.88 0.32 | 2.28 22.88 0.32 | 1.78 11.79 1.6 | 1.78 11.79 1.6 | 1.78 11.79 1.6 | 1.78 11.79 1.6 |
Ramachandran plot | ||||||
 Favored (%)  Allowed (%)  Disallowed (%) | 93.55 6.18 0.27 | 93.55 6.18 0.27 | 97.85 2.15 0 | 97.85 2.15 0 | 97.85 2.15 0 | 97.85 2.15 0 |
