Fig. 7: CA170 treatment increases cytotoxic/exhausted CD8⁺ ratio.

A The UMAP projection of CD8 T cells from two CD45⁺scRNA-seq datasets and CD3⁺scRNA-seq datasets. Each dot corresponds to one single cell and is colored according to its treatment. B The UMAP projection showing the formation of 6 main clusters, including 2 naïve-like CD8⁺ T cells, 2 T_RM-like CD8⁺ T cells, 1 effector population and 1 exhausted population. Each dot corresponds to one single cell and is colored according to its cell type. C Z-score normalized mean expression of selected T cell function-associated genes in each cell cluster. D Stacked percentage bar plot showing the proportion of each cell type in the 5 treatment groups. Colors represent various cell types as shown in (B). E Dotplot showing the statistical comparison of the frequencies of cytotoxic/exhausted CD8⁺ T cells in the different treatment groups. Data are shown as the mean ± SE, n = 4, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs Ctrl. F Z-score normalized mean expression of cytotoxicity-related molecules and inhibitory receptors in Cx3cr1⁺ cytotoxic and exhausted CD8⁺ T cells, respectively. Each column represents a different treatment. G Real-time RT-PCR validation of genes regulated by CA170 in CD4⁺ or CD8⁺ TILs isolated from tumors treated with vehicle or CA170. Data are shown as the mean ± SE of three replicate samples per group, n = 4, *P ≤ 0.05, **P ≤ 0.01 vs Ctrl, one-way ANOVA.