Fig. 6: BRPF1 is related to liver cancer stem cell properties.
From: Bromodomain-containing protein BRPF1 is a therapeutic target for liver cancer
a Upregulation of BRPF1 in CD133 + positive liver cancer stem cells compared to CD133- Huh-7 and PLC cells. cDNA microarray data were extracted from Gene Expression Omnibus: GSE23450 and GSE23451. b GSK5959 reduced the expression of stem cell factors in Huh-7 cells after 24-h treatment. c In all, 10 μM GSK5959 treatment for a week significantly reduced the sphere formation ability of Huh-7 cells in nonadherent culture condition (Scale bar: 0.1 mm). d 10 μM GSK5959 induced cell cycle G1 arrest in MHCC97L cells after 48-h treatment. e 10 μM GSK5959 induced cellular senescence in MHCC97L cells after 5-day treatment, as shown by the β-galactosidase assay (Scale bar: 0.3 mm). Serum-starved cells served as a positive control. Error bars indicate mean ± SD. Data were compared by independent t-test. Results were repeated at least three times. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. mock as indicated.
