Fig. 5: N21Q fibrils exhibit oligomer-like conformation and expose residue F23. | Communications Biology

Fig. 5: N21Q fibrils exhibit oligomer-like conformation and expose residue F23.

From: Identification of transmissible proteotoxic oligomer-like fibrils that expand conformational diversity of amyloid assemblies

Fig. 5

a Dot-blot analysis of IAPP and N21Q oligomers and fibrils. b Immunogold labelling electron microscopy images of IAPP and N21Q fibrils, scale bar: 100 nm. 3× zoom image of the region indicated by the rectangle. a, b Peptides were incubated under quiescent conditions for 15 min (oligomers) and 48 h (fibrils) at 150 µM in 20 mM Tris-HCl buffer, pH 7.4. c FCN fluorescence emission spectra over self-assembly time. Peptides were incubated under quiescent conditions for 48 h at 150 µM in 20 mM Tris-HCl buffer, pH 7.4.

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