Fig. 7: The oligomer-like conformation of N21Q fibrils propagates to IAPP. | Communications Biology

Fig. 7: The oligomer-like conformation of N21Q fibrils propagates to IAPP.

From: Identification of transmissible proteotoxic oligomer-like fibrils that expand conformational diversity of amyloid assemblies

Fig. 7

a–d Self-assembly of IAPP seeded with N21Q pre-assembled fibrils (0.5–5 mol%) was characterized by a ThT fluorescence and ANS fluorescence, b dot-blot analysis with the anti-oligomer A11 antibody, c SS-NMR (CP-DARR) of F23 labelled IAPP, and d FCN fluorescence of the F23FCN IAPP. e Cell viability of INS-1E treated with 50 μM IAPP fibrils seeded with N21Q pre-assembled fibrils. a–e N21Q fibrillar seeds were grown for 48 h (quiescent conditions, 150 µM in 20 mM Tris-HCl buffer, pH 7.4) before being isolated and used to seed the amyloid formation of IAPP under quiescent conditions for 48 h.

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