Fig. 6: Effects of COM-MP secretome on α-SMA level in BHK-21 renal fibroblasts.

BHK-21 cells were incubated in fresh serum-free medium without any treatment (untreated control) or in fresh serum-free medium mixed 1:1 (v/v) with Ctrl-MP secretome or COM-MP secretome for 24 h. a Immunofluorescence staining of α-SMA was done using mouse monoclonal anti-α-SMA as a primary antibody, whereas secondary antibody was conjugated with Alexa Flour 488. Nuclei were stained in blue using Hoechst dye. b Quantitative analysis of fluorescence intensity of α-SMA was obtained from at least 100 cells in ≥10 random HPFs for each sample using NIS-Elements D V.4.11 (Nikon). The data were obtained from three independent experiments using different biological replicates and the bars indicate mean ± SEM (all the source data are presented in Supplementary Data 3). A.U. = arbitrary unit.