Fig. 9: Effects of COM-MP secretome on MMPs activities.

BHK-21 cells were incubated in fresh serum-free medium without any treatment (untreated control) or in fresh serum-free medium mixed 1:1 (v/v) with Ctrl-MP secretome or COM-MP secretome for 24 h. Thereafter, the clarified culture supernatant was subjected to gelatin zymogram assay. a MMP-9 and MMP-2 appeared as transparent bands at approximately 90 kDa and 65-70 kDa, respectively. b, c Absolute fibroblast levels of MMP-9 and MMP–2, respectively, were calculated using Formula 3. The data were obtained from three independent experiments using different biological replicates and the bars indicate mean ± SEM (all the source data are presented in Supplementary Data 6 and Supplementary Data 7, respectively). A.U. = arbitrary unit.