Fig. 1: Profiling how pRB-depletion changes human RPE1 cells. | Communications Biology

Fig. 1: Profiling how pRB-depletion changes human RPE1 cells.

From: Integrated multi-omics analysis of RB-loss identifies widespread cellular programming and synthetic weaknesses

Fig. 1

a Schematic detailing experimental approach and western blot of pRB and Tubulin (TUB) from RPE1 cells infected with DOX-inducible shRNAs targeting Firefly Luciferase (LUC) or pRB (RB-25 and RB-26). b Log2 fold-change of RNA changes in RPE1 cells depleted of pRB (RB25 and RB26), Red dots are statistically significant. c Gene ontology analysis of upregulated mRNAs (Green box, enrichment scores, and FDR p-values) and downregulated mRNAs (Red box, enrichment scores, and FDR p-values). d Graph of genes changed upon pRB-depletion from RPE1 cells that are bound by E2F1 or E2F4/6 from ChIP data. e Log 2 Protein fold-change from RPE1 cells depleted of shRNAs targeting RB using shRNA RB25 and RB26, Red dots are statistically significant. f Gene ontology analysis of upregulated proteins (Green box, enrichment scores, and FDR p-values) and downregulated proteins (Red box, enrichment scores and FDR p-values).

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