Fig. 3: Glucose and purine/pyrimidine metabolism changes in pRB-depleted cells. | Communications Biology

Fig. 3: Glucose and purine/pyrimidine metabolism changes in pRB-depleted cells.

From: Integrated multi-omics analysis of RB-loss identifies widespread cellular programming and synthetic weaknesses

Fig. 3

a Heat map of metabolic changes in LUC, RB25, and RB26 DOX-treated/DOX-untreated with fold changes and adjusted p-value calculations. b Pathway analysis of Glucose metabolism and TCA cycle genes upon pRB-loss. Upregulated metabolites highlighted in yellow, downregulated metabolites shown in green, protein increased in pRB-depleted cells shown in blue and proteins decreased in pRB-depleted RPE1 cells shown in red. c Pathway analysis of Pyrimidine metabolism upon pRB-loss. Upregulated metabolites highlighted in yellow, protein increased in pRB-depleted cells shown in blue. d Pathway analysis of Purine metabolism upon pRB-loss. Upregulated metabolites highlighted in yellow, protein increased in pRB-depleted cells shown in blue. e LCMS data showing relative (pRB (−)/pRB (+)) metabolite changes from RPE1 cells for Glucose pathway metabolites (red bars) and Purine/Pyrimidine metabolites (blue bars) (*p < 0.01). n = 4 biological replicates and a total of 8 technical replicates. Error bars represent standard deviations from these replicates. f RNA and Protein changes in glucose metabolism genes (red circles), purine and pyrimidine metabolism genes (blue circles), and TCA cycle genes (green circles) in RPE1 cells depleted of pRB.

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