Fig. 3: The N-terminal of EBNA2 is necessary for phase separation. | Communications Biology

Fig. 3: The N-terminal of EBNA2 is necessary for phase separation.

From: Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation

Fig. 3

a Diagram of the Full length and truncated EBNA2. b Western blot of scFv-mNeonGreen protein, and full length and truncates of scFv-mNeonGreen EBNA2 protein. c Immunofluorescence images of the truncated EBNA2 in HEK 293 T cells (left). d The droplet counts per area of the mNeonGreen-truncated-EBNA2. Error bars represent SD. e, f Time-lapse images of both mNeonGreen-EBNA2(1-396) and mNeonGreen-EBNA2(1-176). Zoom in shows two truncated EBNA2 fusion events. Scale bars, 5 μm, and zoom in 1 μm.

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