Fig. 5: The N-terminal of EBNA2 is necessary and sufficient to induce ACDs. | Communications Biology

Fig. 5: The N-terminal of EBNA2 is necessary and sufficient to induce ACDs.

From: Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation

Fig. 5

a Immunofluorescence images of the polyX-modified EBNA2 N-terminal. Graphs plotting intrinsic disorder analysis for EBNA2 and the design of the polyX-modified EBNA2 N-terminal (left). The sequence (amino acid from 1 to 176) cloned for subsequent experiments is highlighted with a red bar. Below is a schematic diagram of the polyX-modified EBNA2 N-terminal. FRAP recovery images of the EBNA2(1-176), EBNA2(1-176) + 8E, EBNA2(1-176) + 10Q, EBNA2(1-176) + 10H, and EBNA2(1-176) + 10G condensates (middle). The yellow box highlights the condensate before, during, and after photobleaching. Data are plotted (right) as SD (n = 3). Scale bars, 5 μm. b Representative images show the colocalization of the accessible chromatin domains and mNeonGreen-EBNA2(1-176) (top) or mNeonGreen-EBNA2(1-176) + 8E (bottom) condensates. The experiment was repeated three times independently with similar results. Scale bars, 5 μm. Magnified regions, scale bars, 1 μm.

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