Fig. 1: Postnatal deletion of Fbxw7 leads to changes in bone architecture.
From: FBW7 couples structural integrity with functional output of primary cilia
a Representative three-dimensional reconstitution analysis of the architecture of metaphyseal trabecular bone in the distal femur of 12-week-old Fbxw7f/f or UbcCreERT2; Fbxw7f/f mice treated with 4-Hydroxy-tamoxifen (4OHT). Scale bars: 100 µm. b–g Quantitative μCT analysis of trabecular (b–f) or cortical (g) skeletal parameters: BV/TV (Bone volume fraction) (b), Connectivity density (c), DT-Tb.N (Trabecular number) (d), DT-Tb.Sp (Trabecular separation) (e), DT-Tb.Th (Trabecular thickness) (f) of the metaphyseal trabecular bone or BV/TV of the cortical bone (g) in the distal femur of 12-week-old Fbxw7f/f (n = 10) or UbcCreERT2;Fbxw7f/f (n = 8) mice treated with 4OHT. Data are presented as means ± SEM. Student’s t-test, **p < 0.01, ***p < 0.001. h, i ELISA analysis for bone formation (Mouse Bone-specific alkaline phosphatase, BALP) (h) or bone resorption (Mouse Tartrate-resistant acid phosphatase 5b, TRAcP-5b) (i) markers in the serum of 12-week-old Fbxw7f/f (n = 7) or UbcCreERT2; Fbxw7f/f (n = 7) mice treated with 4OHT. Data are presented as means ± SEM. Student’s t-test, *p < 0.05.
