Fig. 2: Deletion of Fbxw7 reduces cilium incidence in MSCs and MEFs.

a, b Representative images of MSCs isolated from the bone marrow of UbcCre^ERT2; Fbxw7^f/f mice, ex vivo treated with ethanol (mock) or 4OHT in ethanol, serum starved for 24 h and stained for the SSC marker CD106 (green) and primary cilia (acetylated α-tubulin, red) (a). Percent of CD106+ cells with cilia in mock or 4OHT treated cells isolated from four different UbcCre^ERT2; Fbxw7^f/f mice at 4 weeks of age (b). 100 mock-treated and 100 4OHT-treated CD106+ SSCs were analyzed per mouse. Scale bars: 5 µm. Scale bars in insets: 2 µm. Data are presented as means ± SEM. Student’s t-test, *p < 0.05. c–e Representative images of mouse embryonic fibroblasts (MEFs) transfected with GFP and pcDNA3 or Fbxw7-specific sgRNA (sgFbxw7), serum starved for 24 h and stained for primary cilia (acetylated α-tubulin, red) (c). Percent of cilium incidence (d) and ciliary length (e) in MEFs transfected with the indicated constructs. ~100 GFP + MEFs were analyzed for each group per experiment (n = 3) in d. For ciliary length analysis, the number of cells analyzed is indicated at the bottom of each bar in e. Scale bars: 5 µm. Scale bars in insets: 2 µm. Data are presented as means ± SEM. Student’s t-test, *p < 0.05.