Table 1 Estimated expression yield and calculated purified yield for rituximab IgG1 μtp constructs.

From: On-target IgG hexamerisation driven by a C-terminal IgM tail-piece fusion variant confers augmented complement activation

mAb construct

Expressed yield* (mg/L)

Purified yield+ (mg/L)

% of expressed yield

Rituximab IgG1 wild-type

414.3 ± 114.9

245.8 ± 73.5

59.3

Rituximab IgG1 μtp C575S

398.4 ± 8.8

211.3 ± 95.4

53.0

Rituximab IgG1 μtp

315.5 ± 81.0

40.8 ± 20.7

12.9

  1. *Expression yield (mg/L) was calculated post expression by protein G HPLC. +Purified yield (mg/L) was calculated post size-exclusion chromatography. Data shown are mean ± SD of expression and purifications of the three different mAb formats (n = 3 different preparations).
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