Fig. 3: CdGAP promotes cell migration and invasion in CRPC cells. | Communications Biology

Fig. 3: CdGAP promotes cell migration and invasion in CRPC cells.

From: CdGAP promotes prostate cancer metastasis by regulating epithelial-to-mesenchymal transition, cell cycle progression, and apoptosis

Fig. 3

a, b Quantification of transwell migration (a) and invasion (b) assays of CdGAP-depleted PC-3 and 22Rv1 (shCdGAP) cells with corresponding controls (shCon) (n = 3). c, d Representative images from the wound healing assays of CdGAP-depleted PC-3 (c) and 22Rv1 (d) (shCdGAP) cells with corresponding controls (shCon). Scale bar, 400 μm. Quantification of the wound confluence over a period of 27 h (n = 3). e, f Quantification of transwell migration (e) and invasion (f) assays of DU-145, 22Rv1, and LNCaP cells transfected with either empty vector (EV) or GFP-CdGAP (22Rv1, LNcaP: n = 3; DU-145: n = 4). g Adhesion assays of CdGAP-depleted PC-3 (shCdGAP) and control cells (shCon) on fibronectin and collagen type 1 (n = 4). Two-sample unpaired Student’s t test for comparison between two groups. Error bars indicate SEM. ****p < 0.0001 ***p < 0.001; **p < 0.01; *p < 0.05. n.s. not significant.

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