Fig. 5: CdGAP controls a set of EMT, cell cycle, and apoptosis-related genes. | Communications Biology

Fig. 5: CdGAP controls a set of EMT, cell cycle, and apoptosis-related genes.

From: CdGAP promotes prostate cancer metastasis by regulating epithelial-to-mesenchymal transition, cell cycle progression, and apoptosis

Fig. 5

a Volcano plot of the differentially expressed genes between shCdGAP PC-3 and shControl cells. Red dots represent genes with an absolute fold change > 1 (log2FC = 1) and adjusted p-value < 0.01. b Normalized enrichment scores (NES) of significantly enriched and depleted Hallmark gene sets identified via GSEA in shCdGAP vs. shControl cells (p-value < 0.05). c Enrichment plots depicting selected gene sets significantly enriched (apoptosis, EMT) or depleted (G2M Checkpoint) in CdGAP-depleted PC-3 cells. d Top modulated biological processes enriched in CdGAP-depleted cells. e qPCR analyses of the EMT-related genes after CdGAP downregulation. (CDH1, SNAI1, CDH2: n = 4; SNAI2: n = 6). f Immunoblot analysis of the EMT-related proteins after CdGAP downregulation. Tubulin was used as a loading control. Graphs provide a densitometry analysis of the indicated protein levels represented as the fold change relative to control. (Snail1, Slug: n = 4; E-Cadherin: n = 5; N-Cadherin: n = 6). Two-sample unpaired Student’s t test for comparison between two groups (shCon; shCdGAP). Error bars indicate SEM. ****p < 0.0001 ***p < 0.001; **p < 0.01; *p < 0.05.

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