Fig. 8: CdGAP controls metastatic progression. | Communications Biology

Fig. 8: CdGAP controls metastatic progression.

From: CdGAP promotes prostate cancer metastasis by regulating epithelial-to-mesenchymal transition, cell cycle progression, and apoptosis

Fig. 8

a–d Weight, volume, and density of primary tumors and representative photographs of primary tumors collected from control (shCon) or CdGAP-depleted (shCdGAP) PC-3 cells-injected mice at the experimental endpoint (28 days) (shCon: n = 5; shCdGAP: n = 6). Two-sample unpaired Student’s t test for comparison between two groups (shCon; shCdGAP). Error bars indicate SEM. ns: not significant. e Representative images of H&E staining of primary tumors. f Quantification of apoptotic cells in primary tumors by assessing the percentage of cleaved caspase-3 positive cells (shCon: n = 5; shCdGAP: n = 6). Representative images of IHC staining of cleaved caspase-3 in primary tumors. Two-sample unpaired Student’s t test for comparison between two groups (shCon; shCdGAP). Error bars indicate SEM. **p < 0.01. g Representative images of metastases found in control or CdGAP-depleted PC-3 cells-injected mice following ex vivo bioluminescent imaging at the experimental endpoint (28 days). Each mouse was exposed for 4 min after removal of the primary tumor. Yellow circle, intestine; orange circle, testis; red arrow, kidney; green arrows, legs. h Percentage (number; #) of mice with local and distant metastases quantified following ex vivo bioluminescent imaging at the experimental endpoint (28 days). The average number (#) of intestine lesions was quantified in each control and shCdGAP mice with metastases. i Representative images of H&E staining of the kidneys from control (shCon) or CdGAP-depleted (shCdGAP) PC-3 cells-injected mice. Black arrows show tumor lesions. j Violin plots of CdGAP intensity as scored in benign adjacent (BA) and matched tumor (T) tissue cores from the TF123 TMA (n = 282; cytoplasm, p = 1.2 × 10−21; nucleus, p = 0.013; Wilcoxon rank-sum test). k Kaplan–Meier curves of bone metastasis-free survival (10 years) based on CdGAP cytoplasmic staining in tumor cores from the TF123 TMA (p = 0.057). l Univariable analyses revealed that high CdGAP expression in tumor (CdGAP-T) cores are a prognostic factor for progression to bone metastasis (Supplementary Table 2).

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