Fig. 1: Attraction to ascr#8 is sex specific.
From: Distinct neuropeptide-receptor modules regulate a sex-specific behavioral response to a pheromone
a Design of the Single Worm Attraction Assay (SWAA). The outer 40 wells of a 48-well suspension cell culture plate are seeded with NGM agar and a thin lawn of OP50 E. coli. A random block design results in spatial control (light gray), vehicle control (dark gray), and ascaroside (purple) containing wells. Quadrants are recorded for 15 m. (Inset) The structure of ascr#8. b Raw dwell times of males of N2 (blue), him-5 (red), him-8 (purple), osm-3;him-5 (orange) in SWAA. c Transformed log(fold-change) of male dwell time data. d Raw dwell time of hermaphrodites across strains and e log(fold-change) of SWAA data of hermaphrodites. Light gray denotes spatial controls (“S”) (when applicable), dark grey denotes vehicle controls (“V”), colors denote ascr#8 values (“A”). ♂ denotes male data, ⚥ denotes hermaphrodite data. For all figures: Error bars denote SEM. n ≥ 5. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, unless denoted otherwise. For 1b: ++++p < 0.0001, vehicle vs. spatial control.
