Fig. 5: Illustration of 3D-reconstruction of neurons and segmentation of pyramidal cells.
From: Cellular 3D-reconstruction and analysis in the human cerebral cortex using automatic serial sections
A Visualization of the 3D-reconstruction of one layer neurons with a window height of 31 μm. B Zoomed image of the segmented pyramidal and non-pyramidal cells. Scale bar = 20 μm. C Overlay image of the binary segmented image with the gray-scale image of a section. Scale bar = 200 μm. D, E Close-up view of the overlay picture and 3D-reconstruction of pyramidal cells, with yellow lines representing orientations. Scale bar = 15 μm. F Measurement of diameter from the longest cell profile DiaL was utilized to construct 3D spherical objects with their corresponding 3D-reconstructed cell. The graphic indicates that the difference in 3D volume space between the spherical approximation and the 3D-reconstructed item is similar. Scale bar = 20 μm.
