Fig. 3: Nrf2 activation does not affect the expression of pro-inflammatory cytokines in tumor or peri-tumoral tissue.
From: Nrf2 activation does not affect adenoma development in a mouse model of colorectal cancer
a–c mRNA levels for Il1β (a), Il6 (b) Ptgs2 (c), and Il10 (d) in colorectal tumors and peri-tumoral colon tissue of female Gstp−/−: ApcMin/+: Nrf2+/+: Keap1+/+ (WT), Gstp−/−: ApcMin/+: Nrf2−/−: Keap1+/+(Nrf2-KO), and Gstp−/−: ApcMin/+: Nrf2+/+: Keap1flox/flox (Keap1-KD) mice (n = 5–11). Hprt1 was used as the reference gene. e mRNA levels for Nqo1 in tumor vs. peri-tumoral tissues in Gstp−/−: ApcMin/+: Nrf2+/+: Keap1+/+ (WT) and Gstp−/−: ApcMin/+: Nrf2−/−: Keap1+/+ (Nrf2-KO) mice (n = 12). Rplp0 was used as the reference gene. f–h mRNA levels for Gclc (f), Gclm (g), and Hmox1 (h) in tumor vs. peri-tumoral tissues in Gstp−/−: ApcMin/+: Nrf2+/+: Keap1+/+ (WT) and Gstp−/−: ApcMin/+: Nrf2−/−: Keap1+/+(Nrf2-KO) mice (n = 5–6). Rplp0 was used as the reference gene. i Protein levels for Hmox1 in tumor vs. peri-tumoral tissues in Gstp−/−: ApcMin/+: Nrf2+/+: Keap1+/+ (WT) and Gstp−/−: ApcMin/+: Nrf2−/−: Keap1+/+(Nrf2-KO) mice (n = 4). β-actin was used as loading control. *p < 0.05 vs. WT peri-tumor; **p < 0.05 tumor vs. peri-tumor.
