Fig. 9: Chronic pharmacological activation of Nrf2 by TBE-31 does not affect colorectal adenoma formation in Gstp−/−: ApcMin/+ mice.
From: Nrf2 activation does not affect adenoma development in a mouse model of colorectal cancer
a Chemical structure of TBE-31. b mRNA levels for Nqo1 in colons from Gstp−/−: ApcMin/+: Nrf2+/+: Keap1+/+ (WT) mice (n = 3–4) that had been treated with vehicle (0.7% DMSO in corn oil) or the indicated doses of TBE-31, per os, 3 days per week for 1 week, 72h-, 48h- and 48h-apart; colon tissue was harvested 24 h after the last dose. Actb was used as the reference gene. *p < 0.05 vs. vehicle-treated. c–e Tumor multiplicity (c, d) and burden (e) in colons of 20-week-old Gstp−/−: ApcMin/+: Nrf2+/+: Keap1+/+ (WT, black circles) and Gstp−/−: ApcMin/+: Nrf2−/−: Keap1+/+(Nrf2-KO, blue circles) mice (n = 27–30) that had been treated per os with vehicle (0.7% v/v DMSO in corn oil, closed circles) or TBE-31 (5 nmol/g body weight, 3 days per week for 12 weeks, starting at week 8 of age, open circles).
