Fig. 4: Hit 1 blocks aggregation of liposomes and cells with P-cadherin on their surfaces.
From: Regulation of cadherin dimerization by chemical fragments as a trigger to inhibit cell adhesion
a Images of CHO cell line expressing P-cadherin in the presence of 1 mM CaCl2 and 1 mM EDTA and a mock cell line. Scale bars indicate 500 µm. b Representative images of CHO cell line expressing P-cadherin in the presence of 1 mM CaCl2 and increasing concentrations of Hit 1. This experiment was repeated three times, and similar results were obtained. Scale bars indicate 500 µm. c Quantification of cell-aggregation assay using MFI. Hit 1 inhibited the formation of cell aggregation in a dose-dependent manner. N = 3. Error bars show standard deviation. d Liposomes with MEC12 on the surface were incubated with CaCl2 and with (red) or without (black) Hit 1. Controls had no effect on optical density (blue, yellow, and gray traces). A representative plot of optical density at 650 nm is shown as a function of time. N = 3. e The kobs of liposome aggregation was calculated assuming a one-phase association model. N = 3. Error bars show standard deviation. f Normalized area of HCT116 and MCF7 cells in the presence or absence of Hit 1. In order to take cell shape into account, the cell area in the absence of Hit 1 was taken as 100%. N = 3. Error bars show standard deviation. Individual data points are shown in black plots.
