Fig. 1: Localization and phosphorylation of HA-MOP in mouse brain.
From: HA-MOP knockin mice express the canonical µ-opioid receptor but lack detectable splice variants
a Schematic representation of the N-terminally tagged HA-MOP and the C-terminal tail with potential phosphorylation sites (black: antibody detectable phosphorylation sites; light gray: possible phosphorylation sites). b Western blot analysis of brain lysates from HA-MOP mice compared to wild-type animals after immunoprecipitation with anti-HA magnetic beads. Proteins were either immunoblotted with anti-HA antibody (left panel) or UMB-3 (right panel). c Western blot analysis of HA-MOP expression in different organs. Blots are representative of three independent experiments. d 40 µm brain section of an adult HA-MOP mouse (right panel) compared to wild-type (left panel). Slices were stained with Cy3-labeled (550 nm) secondary antibody and pseudo-colored afterward. e Developmental expression of HA-MOP in mouse striatum across different age stages. 40 µm brain slices were stained with anti-HA antibody followed by Cy3-labeled secondary antibody and pseudo-colored afterward. d, e Scale bar = 1000 µm. CPu caudate putamen.
