Table 1 MS analysis of agonist-induced phosphorylation sites in the MOP carboxy-terminus.

From: HA-MOP knockin mice express the canonical µ-opioid receptor but lack detectable splice variants

Peptide sequence

Peptide position

MH + [Da]

# Missed cleavages

Best Mascot ion score

    

Best phosphoRS site probability (%)

 

Phosphosite position

  

Saline

Morphine

Fentanyl

Etonitazene

YIAVCHPVK

166-174

1086.5765

0

41

43

45

47

EFCIPTSSTIEQQNSAR

349-365

1967.9127

0

48

83

74

75

EFCIPTSSTIEQQNSAR

349-365

2047.8791

0

  

60

53

 

S363

    

100

100

IRQNTREHPSTANTVDR

366-382

2074.9778

2

  

44

43

 

T370

    

100

100

IRQNTREHPSTANTVDR

366-382

2154.9441

2

  

22

38

 

T370; S375

    

99.99; 49.79

100; 95.64

IRQNTREHPSTANTVDR

366-382

2154.9441

2

 

20

22

22

 

T370; T376

   

98.78; 89.93

99.99; 92.7

100; 92.96

IRQNTREHPSTANTVDR

366-382

2234.9105

2

   

25

 

T370; S375; T376

     

100; 99.99; 99.91

QNTREHPSTANTVDR

368-382

1805.7926

1

 

29

21

42

 

T370

   

100

100

100

QNTREHPSTANTVDR

368-382

1885.7590

1

 

14

13

14

 

T370; S375

   

98.87; 99.85

100; 99.3

99.89; 99.08

TNHQLENLEAETAPLP

383-398

1776.8763

0

38

70

58

60

  1. List of MOP receptor peptides and phosphopeptides identified by nanoLC–MS/MS and their corresponding Mascot ion score in each condition. Below the Mascot score, phosphoRS site probability (%) is given for each phosphorylated amino acid position (bold numbers). Positions are indicated for the canonical protein (P42866).
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