Table 2 Plasmids used in this study.
Plasmids | Description | Reference or source |
|---|---|---|
pRS416 | Yeast shuttle vector | |
p416ADH | Yeast shuttle vector | |
p425ADH | Yeast shuttle vector | |
pET48b(+) | Trx-His tagged protein expression vector | Merck Millipore |
pET49b(+) | GST-tagged protein expression vector | Merck Millipore |
pET50b(+) | His-Nus-His tagged protein expression vector | Merck Millipore |
pTBK1 | Intein-CBD tagged protein expression vector | New England Biolabs |
pMH330 | p416ADH-PIB2 | This study |
pMH334 | p416ADH-pib2 (Δ1-115) | This study |
pMH342 | pRS416-PIB2 | This study |
pMH410 | p416ADH-pib2 (Δ1-303) | This study |
pMH417 | pET49b(+)-pib2 (304-635) | This study |
pMH422 | pET49b(+)-pib2 (304-620) | This study |
pMH426 | pET49b(+)-pib2 (440-635) | This study |
pMH433 | pRS415-PIB2 | This study |
pMH497 | pRS416-pib2(P337A) | This study |
pMH498 | pRS416-pib2(R341A) | This study |
pMH499 | pRS416-pib2(L340A) | This study |
pMH508 | Same as pMH417 except PIB2(V626A) | This study |
pMH510 | p416ADH-pib2(P337A) | This study |
pMH512 | p416ADH-pib2(L340A) | This study |
pMH514 | p416ADH-pib2(R341A) | This study |
pMH553 | Same as pMH417 except PIB2(P627S) | This study |
pMH554 | Same as pMH417 except PIB2(A628T) | This study |
pMH555 | Same as pMH417 except PIB2(N629S) | This study |
pMH568 | Same as pMH417 except (R341A, V626A) | This study |
pMH606 | pET48b(+)-pib2(304-533, Δ356-384) | This study |
pMH610 | pET50b(+)-pib2(304-533, Δ356-384) | This study |
pMH649 | pTBK1-Trx-His-pib2(304-635) | This study |
pKY11-1 | Same as pMH334 except PIB2(V626A) | This study |
pKY14-1 | Same as pMH334 except PIB2(P627S) | This study |
pKY24-1 | Same as pMH417 except PIB2(A628T) | This study |
pKY21 | Same as pMH334 except PIB2(N629S) | This study |
pKY95 | Same as pMH342 except pib2(Δ356-384) | This study |
