Fig. 1: Benzopyrimido-diazipinone scaffolds and their binding to DCLK1-KD.
From: Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1
a DCLK1 domain architecture highlighting the DCX domains at the N-terminal region and the kinase domain with its C-terminal regulatory tail (CT) at the C-terminal region. The N-terminal and the C-terminal regions are connected by the serine/proline (S/P) rich linker. Chemical structures of NVP-TAE684 and benzopyrimido-diazipinone scaffolds. R1, R2, R3, and R4 positions previously described28 are labelled and coloured. b Change in melting temperature (ΔTm) of DCLK1-KD as a function of the concentration of XMD8-85, DCLK1-IN-1 and DCLK1-NEG. Change in the ΔTm was calculated from the melting profiles shown in Supplementary Fig. 1. Each data point represents the calculated ΔTm calculated for each inhibitor concentration; shown in duplicate experiments are shown.
