Fig. 1: Deletion of Hif1α increases total tumor burden while slowing primary tumor growth.

a Example genotyping gel of Cre, floxed Hif1α, and PyMT from Hif1α^f/f PyMT⁺, Hif1α^−/− PyMT⁺, and Hif1α^f/w PyMT⁻ mice. “+ Ctrl” product indicates an internal positive control. b Survival analysis of Hif1α^f/f PyMT⁺ and Hif1α^−/− PyMT⁺ mice where end point represents sacrifice due to tumor size reaching collection threshold. Log-rank test. c–e Comparison of the age at sacrifice, total tumor burden at sacrifice, and number of tumors collected per mouse. Two-tailed Mann–Whitney test. f–h Quantitative PCR analysis of Hif1α, Hif2α, and Vegfa expression compared to B2m from whole-tumor homogenate RNA. Expression is normalized to the mean of the f/f control group. Two-tailed Mann–Whitney test. i The average pimonidazole staining intensity for each mouse across multiple images from a single tumor. Two-tailed Mann–Whitney test. j Representative images of pimonidazole staining, taken with ×10 and ×40 objectives. The ×40 field is denoted with a dashed box in the ×10 view. Scale bars represent 500 μm in ×10 fields, and 200 μm in ×40 fields. Negative controls lacked the primary antibody incubation step. Graphs represent mean per group and error bars represent s.e.m. n = 19 (or 16 in i) Hif1α^f/f PyMT⁺ mice and n = 21 Hif1α^−/− PyMT⁺ mice.