Fig. 2: Deletion of Hif1α in the mammary fat pad reduces trabecular bone volume.

a Representative 3D renderings of microCT scans of the proximal metaphysis of the right tibia. b–e Quantification of bone volume as a percentage of total volume, trabecular number, trabecular spacing, and trabecular thickness. Two-tailed Mann–Whitney test against corresponding f/f control. f Representative H&E-stained images of the proximal metaphysis of the tibia taken with ×4 and ×20 objectives. The ×20 field is denoted with a dashed box in the ×4 view. Scale bars represent 2.0 mm in ×4 view and 200 μm in the ×20 view. g H&E-stained images of the one histologically detectable bone metastatic lesion, identified in the distal region of the tibia from Mouse 27. Lower-magnification images taken with ×4 (i), ×20 (ii) objectives, while highest magnification image (iii) is taken at ×600 magnification. Scale bars represent 2.0 mm in ×4 view, 200 μm in the ×20 view, and 25 μm in ×600 view. Area of higher magnification is indicated in lower magnification images with a dashed box. Tumor area is labeled T and indicated by dashed circle. Atypical cells denoted with arrowheads. Graphs represent mean per group and error bars represent s.e.m. n = 19 Hif1α^f/f PyMT⁺ mouse tibiae, n = 21 Hif1α^−/− PyMT⁺ mouse tibiae, n = 16 Hif1α^f/f PyMT⁻ mouse tibiae, and n = 11 Hif1α^−/− PyMT⁻ mouse tibiae.