Fig. 3: Deletion of Hif1α decreases bone dissemination while increasing lung metastasis.
a The percentage of EpCAM+ cells, out of the total number of live cells, detected by flow cytometric analysis of left hindlimb bone marrow. Numbers are normalized to the total tumor weight of each mouse. Two-tailed Mann–Whitney test. b, c Quantitative PCR analysis of Pymt transcript compared to Hmbs from right femur or spinal midsection, respectively. Pymt expression was then normalized to the total tumor weight of each mouse. Two-tailed Mann–Whitney test. d, e Metastatic lesion number or area detected by histological analysis of H&E-stained sections from the left lung. Numbers are normalized to the total tumor weight of each mouse. Two-tailed Mann–Whitney test. f Comparison of the proportion of mice from each group that had any detectable pulmonary lesions. Fisher’s exact test. g Representative images of H&E-stained lung sections. Scale bar in sub-gross photomicrographs is 500 μm. Scale bar in high-power micrographs is 100 μm. The high-power image field is denoted with a dashed box in the sub-gross view. Tumor area is denoted with a dashed oval in the high-power images (labeled T). Additional metastatic lesions in the sub-gross view are denoted with arrows. Graphs represent mean per group and error bars represent s.e.m. n = 19 Hif1αf/f PyMT+ mice, n = 21 Hif1α−/− PyMT+ mice.
