Fig. 5: Deletion of Hif2α decreases tumor dissemination to the bone but not to the lung.

a The percentage of EpCAM⁺ cells, out of the total number of live cells, detected by flow cytometric analysis of left hindlimb bone marrow. Two-tailed Mann–Whitney test. n = 10 Hif2α^f/f PyMT⁺ or Hif2α^−/− PyMT⁺ mice. b, c Quantitative PCR analysis of Pymt transcript compared to Hmbs from right femur or spinal midsection, respectively. Two-tailed Mann–Whitney test. d, e Metastatic lesion number or area detected by histological analysis of H&E-stained sections from the left lung. Two-tailed Mann–Whitney test. f Comparison of individual lesion areas detected from histological inspection of left lung sections. Two-tailed Mann–Whitney test. n = 96 individual Hif2α^f/f metastatic lung lesions, n = 35 individual Hif2α^−/− metastatic lung lesions. g Comparison of the proportion of mice from each group that had any detectable pulmonary lesions. Fisher’s exact test. h, i Quantitative PCR analysis of Pymt transcript compared to B2m from the right lung or brain, respectively. Two-tailed Mann–Whitney test. Graphs represent mean per group and error bars represent s.e.m. For b–e, g–i, n = 19 Hif2α^f/f PyMT⁺ mice, n = 18 Hif2α^−/− PyMT⁺ mice.