Fig. 5: Blocking HLA-DR abrogates TLR4 surface expression in T cells.
a Representative plot and cumulative graph (n = 99 RA patients) of the frequency of HLA-DR+TLR4+ T cells. b Representative plots and cumulative graph (n = 99 RA patients) of the frequency of TLR4 expression by HLA-DR+ T cells. c Representative plots and cumulative graph (n = 99 RA patients) of the frequency of HLA-DR expression by TLR4+ T cells. d Correlation between HLA-DR and TLR4 MFIs in TLR4+ T cells (n = 99 RA patients). e Representative plots and cumulative graph (n = 17 RA patients) of the frequency of TLR4+ T cells after incubating FACS-purified CD4+ T cells with a blocking antibody to HLA-DR for 18 hours. Data are presented as mean ± SD, for parametric statistical tests, or median ± IQR, for non-parametric statistical tests. Sample normality distribution was tested by using D’Agostino & Pearson normality test. P values ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 were determined by (d) Spearman correlation and (e) Wilcoxon matched-pairs rank test. Effect size measures +++high, ++medium, +small were determined by (d) rs – Spearman’s correlation coefficient and (e) r – correlation coefficient r.
