Fig. 6: Neuronal apoptosis in the cerebral cortex of the rat neonatal encephalopathy models.
From: Neuronal let-7b-5p acts through the Hippo-YAP pathway in neonatal encephalopathy
a Images of neuronal cells (NeuN positive, green) that can undergo apoptosis (cleaved caspase-3 positive, red) and coexpression is shown (yellow) in the cerebral cortex of control (arrowheads indicate non-neuronal apoptotic staining), hypoxic-ischaemic in the ipsilateral (arrows indicate neuronal apoptotic staining) and contralateral region (arrows indicate neuronal apoptotic staining), and intrauterine inflammation model (arrows and arrowheads indicate neuronal and non-neuronal apoptotic staining, respectively). b, c Graphs (b) show the statistical significance in cleaved caspase-3 apoptosis and (c) show no statistical significance in neuronal cell counts co-expressing cleaved caspase-3. Error bars represent mean with SEM (n = 3–6 per group). ns not significant; ** and *** denote significant p < 0.01 and p < 0.001, respectively, using ANOVA with Dunnett’s multiple comparisons test. Scale bar = 50 μm. CTL control, HI hypoxic-ischaemic, ipsi ispsilateral side, contra contralateral side, IUI intrauterine inflammation.
