Fig. 8: Neuronal expression of YAP in the cerebral cortex of the rat neonatal encephalopathy models.
From: Neuronal let-7b-5p acts through the Hippo-YAP pathway in neonatal encephalopathy
a Images of NeuN positive neuronal cells (green) with YAP (red) and coexpression is shown (yellow) in the cerebral cortex of control, hypoxic-ischaemic in the ipsilateral (arrows indicate YAP positive neurones) and contralateral region (arrows indicate YAP positive neurones), and intrauterine inflammation model (arrows indicate YAP positive neurones). b Neuronal cytoplasmic YAP positive immunostaining is determined by strong coexpression of NeuN and YAP. c Nuclear YAP positive immunostaining is determined by strong coexpression of YAP with Hoechst staining in the nucleus. d–f Graphs showing a significant increase in the neuronal/nuclear ratio (d), significant increase in neuronal (e), but no changes in nuclear (f) YAP expression. Error bars represent mean with SEM. n = 3–6 per group. ns not significant; ** and *** denote significant p < 0.01 and p < 0.001, respectively, using ANOVA with Dunnett’s multiple comparisons test. Scale bar = 50 μm for (a) and 10 μm for (b, c). CTL control, HI hypoxic-ischaemic, ipsi ispsilateral side, contra contralateral side, IUI intrauterine inflammation.
