Fig. 1: Molecular classification of hepatocellular carcinoma.
From: Genomic characterization of rare molecular subclasses of hepatocellular carcinoma
a The Spearman correlation to the median expression of CCA tumors (n = 36) was calculated for each TCGA CCA/HCC tumor (n = 410). HCC samples within ±1 standard deviation of the mean CCA Spearman correlation (dashed line) were defined at CCA-Like. b The CCA/HCC dataset was correlated to microdissected normal bile duct (NBD) (n = 6) or normal liver (n = 59) from Andersen et al. A NBD vs. Liver score was calculated by subtracting the correlation to normal liver from the correlation to normal bile duct55. Boxes represent the IQR with the median represented by the bolded bar. Error bars represent Q1/Q3 ± 1.5*IQR. c Single-cell RNA seq data from Yang et al. was used to correlate the CCA/HCC samples to hepatoblasts (E10.5, n = 54), hepatocytes (E17.5, n = 34) or cholangiocytes (E17.5, n = 34). PCA was performed on Yang et al. to visual variance across the samples then the correlation was calculated between the median expression of day E10.5 and E17.5 (hepatocytes and cholangiocytes) samples to the CCA/HCC dataset. HCC samples in the upper tertile of correlation to hepatoblasts and not prior classified as CCA-Like, were defined as Blast-Like (n = 66). Murine embryo images were obtained from http://repo.mouseimaging.ca/repo/4D_embryo_atlases_M_Wong/.
