Fig. 3: Genes involved in the Taxol biosynthetic pathway.
From: Taxus yunnanensis genome offers insights into gymnosperm phylogeny and taxol production
a Transcriptomic analysis of genes involved in the Taxol biosynthetic pathway. The FPKM was calculated to evaluate the expression level of each gene. T1βOH and T9αOH represent the enzymes responsible for C-1 and C-9 oxidation that are currently unverified and which are presumed to belong to CYP450 gene family. b Phylogenetic tree of the CYP725A gene sub-family in T. yunnanensis, S. giganteum, P. menziesii and G. biloba. Genes from the four different plants are labeled in different colors, Blue, T. yunnanensis; Pink, P. menziesii; Red, S. giganteum; Green, G. biloba. c Arrangement and chromosomal positions of three Taxol gene clusters on chromosome 12 (chr12). d Heat maps of gene expression of CYP725A genes located on chromosome 12. The average expression profiles of three replicates of different tissues of T. yunnanensis were used to make the heat map. Color scale represents log2-transformed FPKM (expected number of fragments per kilobase of transcript sequence per millions base pairs sequenced) values. The gradual change of the color indicates the different expression levels of genes, white indicating low transcript abundance and red indicating high levels of transcript abundance.
