Fig. 4: Administration of naphthofluorescein attenuates the interaction between Mint3 and FIH-1 and the expression of HIF-1 target genes in tumour xenografts.
From: Pharmacological inhibition of Mint3 attenuates tumour growth, metastasis, and endotoxic shock
a Body weight of mice with vehicle or naphthofluorescein (Naph; 100 mg/kg b.w.) injection (n = 6 per group). b Administration of Naph attenuates the interaction between Mint3 and FIH-1 in tumours. Tumours of HT1080 cells expressing FLAG-tagged FIH-1 were collected from mice injected vehicle or Naph (n = 5 per group) 3 h after the injection. FLAG-tagged FIH-1 was immunoprecipitated (IP FLAG) from tumour lysates, and precipitates were analysed by western blotting using the indicated antibodies. c–i Expression of HIF-1 target genes (c–g), HIF1A (h), and 18S rRNA (i) in tumours of HT1080 cells 6 h after Naph injection. Error bars indicate the SD (n = 10). Data were analysed using the Mann–Whitney U test.
