Fig. 6: Naphthofluorescein attenuates host Mint3-mediated lung metastasis.

a Schematic illustration of the experimental lung metastasis assay of B16F10 cells. b Photos of metastatic lungs from vehicle- or naphthofluorescein-treated (Naph; 100 mg/kg b.w.) mice. Scale bars = 1 cm. c Number of metastatic foci of B16F10 cells in the lungs from mice treated with vehicle or Naph at the indicated amount (n = 6 mice per group). d Number of metastatic foci of B16F10 cells in the lungs from mice treated with Naph at the indicated dose (n = 5–6 mice per group). e Photos of metastatic lungs from vehicle- or Naph-treated WT and Mint3 KO mice. Scale bar = 1 cm. f Number of metastatic foci of B16F10 cells in the lungs from vehicle- or Naph-treated (100 mg/kg b.w.) WT and Mint3 KO mice (n = 6 mice per group). g Flow cytometric analysis of inflammatory monocytes (IMs) in the lungs at 4 h after intravenous injection of B16F10 cells into vehicle- or Naph-treated (100 mg/kg b.w.) mice (n = 6 per group). h Immunostaining analysis of E-selectin (red) expression in the lungs of vehicle- or Naph-treated mice 6 h after intravenous injection with fluorescein-labelled B16F10 cells (green). Nuclei are stained with Hoechst 33342 (blue). (Left) Representative photos of lungs from vehicle-treated mice. (Right) Quantitative analysis of E-selectin-positive cells in the lungs (n = 9, from three tumours per group). In (c), (d), and (f−h), data are presented as the mean ± SEM. Data were analysed using the Mann–Whitney U test. **p < 0.01. NS not significant.