Fig. 4: UCP1 expression in each seasons and regions. | Communications Biology

Fig. 4: UCP1 expression in each seasons and regions.

From: Adipose gene expression profiles reveal insights into the adaptation of northern Eurasian semi-domestic reindeer (Rangifer tarandus)

Fig. 4

The western blots and relative expression of UCP1 from a–c reindeer prescapular and d–f perirenal adipose tissue total proteins. The upper blots on the left side show UCP1 content in prescapular adipose tissue a in spring in Even reindeer (n = 3) and Finnish reindeer (n = 5), b in winter in Even reindeer (n = 3) and Finnish reindeer (n = 5), and the lower graph shows c their relative expression levels. The upper blots on the right side show UCP1 content in perirenal adipose tissue d in spring in Even reindeer (n = 3) and Finnish reindeer (n = 4), e in winter in Even reindeer (n = 3) and Finnish reindeer (n = 5), and the lower graph shows f their relative expression levels. Samples contained 50 µg of total protein per lane, except the reindeer BAT (brown adipose tissue; BAT and BATr) samples with 5 µg and mouse BAT (BATm) with 1 µg of protein. Alpha tubulin was used as a loading control. The relative expression of UCP1 was normalized to alpha tubulin and presented as mean arbitrary units (a.u.) ± SD (c, f). Significant differences between seasons are indicated with a bar and an asterisk (*p < 0.05).

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