Fig. 5: COX4 expression in each seasons and regions. | Communications Biology

Fig. 5: COX4 expression in each seasons and regions.

From: Adipose gene expression profiles reveal insights into the adaptation of northern Eurasian semi-domestic reindeer (Rangifer tarandus)

Fig. 5

The western blots and relative expression of COX4 from a–c reindeer muscle (M. gluteobiceps femoris) and d–f liver mitochondrial proteins. The upper blots on the left side show COX4 content in the muscle a in spring in Even reindeer (n = 3) and Finnish reindeer (n = 5), b in winter in Even reindeer (n = 3) and Finnish reindeer (n = 5), and the lower graph shows c their relative expression levels. The upper blots on the right side show COX4 in the liver d in spring in Even reindeer (n = 3) and Finnish reindeer (n = 5), e in winter in Even reindeer (n = 3) and Finnish reindeer (n = 5), and the lower graph shows f their relative expression levels. Samples contained 10 µg of mitochondrial protein per lane, except the reindeer BAT (brown adipose tissue) sample with 1 µg of mitochondrial protein. Mitochondrial ATP synthase (ATP5A) was used as a loading control. The relative expression of COX4 was normalized to ATP5A and presented as mean arbitrary units (a.u.) ± SD (c, f). Significant difference between seasons is indicated with a bar and asterisks (**p ≤ 0.01).

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