Fig. 1: Regulation of Smoc1 and Smoc2 expressions by Runx2 and Bmp2.
From: Smoc1 and Smoc2 regulate bone formation as downstream molecules of Runx2
a, b Primary limb bud cells were infected with Venus (control), Runx2, or Bmp2 adenoviruses. At 2, 4, or 6 days after infection, total RNA was extracted from the cells and Smoc1 (a) and Smoc2 (b) mRNA expressions were determined by RT-qPCR (n = 6–10, biologically independent samples). c–f Primary osteoblasts isolated from mouse calvariae were infected with Venus or DN-Runx2 adenoviruses. At 3 days after infection, total RNA was extracted and Osterix (c), Osteocalcin (d), Smoc1 (e), and Smoc2 (f) mRNA expressions were determined by RT-qPCR (n = 4, biologically independent samples). g, h Limb bud cells isolated from WT or Runx2 KO littermate mice were incubated with or without Bmp2 (500 ng/mL) for 4 days. The total RNA was extracted from the cells and Osterix (g) and Osteocalcin (h) mRNA expressions were determined by RT-qPCR (n = 5–8, biologically independent samples). i, j Limb bud cells isolated from WT or Runx2 KO littermate mice were infected with Venus or DN-Runx2 adenovirus, followed by incubation with or without Bmp2 (500 ng/mL). After 4 days of incubation, Smoc1 (i), and Smoc2 (j) mRNA expressions were determined by RT-qPCR (Smoc1: n = 14, Smoc2: n = 3, biologically independent samples). Values are the mean ± SE.
