Fig. 5: Delayed endochondral bone formation in Smoc1/2 DKO mice.
From: Smoc1 and Smoc2 regulate bone formation as downstream molecules of Runx2
Smoc1+/−;Smoc2−/− mice were mated, and Smoc1+/+;Smoc2−/− and Smoc1−/−;Smoc2−/− littermate mice at E15.5 were examined by hematoxylin-eosin (a) and von Kossa (b) staining (n = 4). c In situ hybridization analyses using antisense probes against Col1a1, Col2a1, Pthr1, Col10a1, Mmp13, Osterix, and Runx2. Scale bars: 500 μm (n = 4, biologically independent animals). d The total RNA was extracted from the tibia of Smoc1+/+;Smoc2−/− or Smoc1−/−;Smoc2−/− littermate mice at E15.5. The Runx2, Osterix, Col10a1, and Mmp13 expression levels were determined by RT-qPCR (Smoc1+/+;Smoc2−/−: n = 4, Smoc1−/−;Smoc2−/−: n = 3, biologically independent animals). Values are the mean ± SE.
