Fig. 6: Deletion of SEP-yp1 influences the translocation of T3SS. | Communications Biology

Fig. 6: Deletion of SEP-yp1 influences the translocation of T3SS.

From: Proteogenomic discovery of sORF-encoded peptides associated with bacterial virulence in Yersinia pestis

Fig. 6

HeLa cells were infected with Y. pestis strains for 2 h. After removing the media and thoroughly washing the wells, the infected cells were lysed by Triton X-100 buffer, since it is unable to break the cell wall of bacteria. The mixture was centrifuged to separate the lysate and the cell debris pellet. The samples were immunoblotted. Each assay has three biological replicates.

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