Fig. 3: Importance of the interaction between Zfhx4 and Osterix for late-stage endochondral ossification.
From: Zfhx4 regulates endochondral ossification as the transcriptional platform of Osterix in mice
a Physical interaction of Zfhx4 with Osterix. Coimmunoprecipitation analysis of lysates of 293FT cells transfected with Flag-Zfhx4, 6xMyc-Osterix, or both is shown. Closed arrows: 6xMyc-Osterix, Open arrow: Flag-Zfhx4 (IP immunoprecipitation, WB western blotting). b Colocalization of Flag-Zfhx4 (red) with Venus-Osterix(green) in SW1353 cells. Scale bar = 5 μm. c Skeletal preparations of whole bodies, forelimbs, and hindlimbs of E16.5 wild-type (WT), Zfhx4+/−;Osterix+/−, Zfhx4−/−, and Zfhx4−/−;Osterix+/− littermates stained with Alcian blue and alizarin red. Scale bar = 1 mm. Right panels show higher magnification images of the forelimbs and hindlimbs shown in the left panel. d–f Paraffin-embedded sections of E16.5 WT, Osterix+/−, Zfhx4+/−;Osterix+/−, Zfhx4−/−, and Zfhx4−/−;Osterix+/− littermates subjected to hematoxylin and eosin (HE) staining (d), von Kossa staining (e), and immunofluorescence staining with an anti-Mmp13 antibody (f). Scale bar = 500 μm.
