Table 1 List of sequences of Taqman probe sets for real-time RT-PCR experiments.
From: Zfhx4 regulates endochondral ossification as the transcriptional platform of Osterix in mice
Zfhx4 | Sense | 5’-TGGCCCCACCAAATCTTTGC-3’ |
|---|---|---|
|  | Anti-sense | 5’-TGGCTCGGTCTGCTGTCC-3’ |
Probe | 5’-TCCACCACCACCTCCTCCTCCTCCA-3’ | |
Sox9 | Sense | 5’-CCTTCAACCTTCCTCACTACAGC-3' |
Anti-sense | 5’-GGTGGAGTAGAGCCCTGAGC-3' | |
Probe | 5’-CCGCCCATCACCCGCTCGCAATAC-3' | |
Ihh | Sense | 5’-GACTCATTGCCTCCCAGAACTG-3' |
Anti-sense | 5’-CCAGGTAGTAGGGTCACATTGC-3' | |
Probe | 5’-CCACAGCCAGCCTGGACATCCCGA-3' | |
Runx2 | Sense | 5’-CTCCTTCCAGGATGGTCCCA-3' |
Anti-sense | 5’-CTTCCGTCAGCGTCAACACC-3' | |
Probe | 5’-CACCACCTCGAATGGCAGCACGCT-3' | |
Osterix | Sense | 5’-AGCGACCACTTGAGCAAACAT-3' |
Anti-sense | 5’-GCGGCTGATTGGCTTCTTCT-3' | |
Probe | 5’-CCCGACGCTGCGACCCTCCC-3' | |
Aggrecan | Sense | 5’-TCACTGTTACCGCCACTTTCC-3' |
Anti-sense | 5’-TGCTGCTCAGATGTGACTGC-3' | |
Probe | 5’-ACCGTCTCTCCGCATCCACCCAGG-3' | |
Col2a1 | Sense | 5’-CCTCCGTCTACTGTCCACTGAG-3' |
Anti-sense | 5’-TGGAGCCCTGGATGAGCAAG-3' | |
Probe | 5’-TGAGGTTGCCAGCCGCTTCGTCCA-3' | |
Col10a1 | Sense | 5’-GCCAAGCAGTCATGCCTGAT-3' |
Anti-sense | 5’-GACACGGGCATACCTGTTACC-3' | |
Probe | 5’-AGCACTGACAAGCGGCATCCCAGA-3' | |
Mmp13 | Sense | 5’-GGTTATGACATTCTGGAAGGTTATCC-3' |
Anti-sense | 5’-CGTGGTTCTCAGAGAAGAAGAGG-3' | |
Probe | 5’-CCCGTGTTCTCAAAGTGAACCGCAGCG-3' | |
Alp | Sense | 5’-ATCTTTGGTCTGGCTCCCATG-3' |
Anti-sense | 5’-TTTCCCGTTCACCGTCCAC-3' | |
Probe | 5’-TGAGCGACACGGACAAGAAGCCCTT-3' | |
Osr1 | Sense | 5’-CCACCTACGGGACCACAGATATA-3' |
Anti-sense | 5’-CGGGACTGGCAGAATCCTTT-3' | |
Probe | 5’-CCACACTCTTGACACTTGAAAGGCTTCTCT-3' | |
Osr2 | Sense | 5’-CACCTACGGGATCACAGGTATATC-3' |
Anti-sense | 5’-GCAGCTGTAGGGCTTGATGT-3' | |
Probe | 5’-ACTGACAGAATCCTTTCCCACACTCCTGAC-3' | |
Fgf10 | Sense | 5’-CCTGGAGATAACATCAGTGGAAATC-3' |
Anti-sense | 5’-TCCTCTATTCTCTCTTTCAGCTTACA-3' | |
Probe | 5’-CCGTCAAAGCCATCAACAGCAACTATTACT-3' | |
Msx1 | Sense | 5’-AAGATGCTCTGGTGAAGGCC-3' |
Anti-sense | 5’-TCTTGTGCTTGCGTAGGGTG-3' | |
Probe | 5’-CGTGGATGCAGAGTCCCCGCTTCTC-3' | |
β-actin | Sense | 5’-TTAATTTCTGAATGGCCCAGGTCT-3' |
Anti-sense | 5’-ATTGGTCTCAAGTCAGTGTACAGG-3' | |
Probe | 5’-CCTGGCTGCCTCAACACCTCAACCC-3' |
