Fig. 6: DLST depletion reduces migration, tumor burden, and invasion of human DLST-dependent TNBC cells.

a The representative images (top) and quantitative data (bottom) of transwell cell migration assays for BT-549 and MDA-MB-231 cells in the presence or absence of DLST knockdown: 8–13 individual images from triplicated experiments were quantified for each group. Scale bar = 20 μm. b Overlay of brightfield and RFP channel images of zebrafish embryos transplanted with RFP+ BT-549 cells at 4 days of post-transplantation with or without NAC treatment (top panels). Quantification of zebrafish xenografts with tumor cell invasion (bottom, left) and burden (bottom, right): the average data from each experimental triplicate (23–29 fish per group) were presented as an individual dot. Tumor cell invasion was determined for the percentage of zebrafish xenografts that have tumor cells migrated away from the original injection site (bottom, left). Tumor burden was quantified based on the fluorescence intensity using ImageJ 1.52a (bottom, right). Scale bar = 0.5 mm. Data in (a, b) presented as mean ± s.e.m. *P ≤ 0.05, ***P ≤ 0.001. Statistical differences were calculated using one-way ANOVA.